Actin Alpha 2 Downregulation Inhibits Neural Stem Cell Proliferation and Differentiation into Neurons through Canonical Wnt/β-Catenin Signaling Pathway

Figure 4

ACTA2 downregulation attenuated NSC differentiation into neurons, while facilitating NSC differentiation into astrocytes and oligodendrocytes. (a) Representative immunostaining images depicting the percentage of β-III-Tubulin⁺ cells in each group on day 10. Cell nuclei were counterstained with DAPI in blue. Scale bar: 50 μm. (b) Statistical analysis illustrating the percentage of β-III-Tubulin⁺ cells from (a). ; NS: no statistical difference. (c) Typical immunostaining images illustrating the percent of Olig2⁺ cells in each group on day 10. Cell nuclei were counterstained with DAPI in blue. Scale bar: 50 μm. (d) Bar graph demonstrating the percentage of Olig2⁺ cells from (a). ; NS: no statistical difference. (e) Representative immunostaining images demonstrating the percent of GFAP⁺ cells in each group on day 10. Cell nuclei were counterstained with DAPI in blue. Scale bar: 50 μm. (f) Bar chart summarizing the percentage of GFAP⁺ cells from (a). ; NS: no statistical difference.

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