Actin Alpha 2 Downregulation Inhibits Neural Stem Cell Proliferation and Differentiation into Neurons through Canonical Wnt/β-Catenin Signaling Pathway

Figure 7

CHIR99012 reinforced the differentiation of NSC into neurons, while suppressed NSC differentiation into oligodendrocytes and astrocytes. (a) Representative immunostaining images illustrating the percentage of β-III-Tubulin⁺ cells with the addition of CHIR99012. Cell nuclei were counterstained with DAPI in blue. Scale bar: 50 μm. (b) Statistical analysis demonstrating the percentage of β-III-Tubulin⁺ cells from (a). . (c) Typical immunostaining images depicting the percentage of Olig2⁺ cells with administration of CHIR99012. Cell nuclei were counterstained with DAPI in blue. Scale bar: 50 μm. (d) Bar graph demonstrating the percentage of Olig2⁺ cells from (c). . (e) Representative immunostaining images depicting the percentage of GFAP⁺ cells with treatment of CHIR99012. Cell nuclei were counterstained with DAPI in blue. Scale bar: 50 μm. (f) Bar chart summarizing the percentage of GFAP⁺ cells from (e). .

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