Dysregulation of EZH2/miR-138-5p Axis Contributes to Radiosensitivity in Hepatocellular Carcinoma Cell by Downregulating Hypoxia-Inducible Factor 1 Alpha (HIF-1α)

Figure 6

RNA-seq and verification. (a) Venn diagram showing the overlap of differentially expressed genes in two pair-wise comparisons (miR-138-5p mimic VS NC and HepG2-shEZH2 VS vector). (b–c) Heat maps of representative up and downregulated genes from HepG2 RNA-seq results. (d–e) The significant pathways enriched from the critical differential genes in KEGG analysis. (f) Network interaction diagram of representative and downregulated genes, EZH2, EMT, and HIF-1α-related genes from RNA-seq results. (g) In HepG2 and Hep3B cells, the expression detection of HIF-1α mRNA after transfection of miR-138-5p mimic by RT-qPCR.

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