Exosomal CXCL14 Contributes to M2 Macrophage Polarization through NF-κB Signaling in Prostate Cancer

Figure 3

CXCL14 knockdown inhibited M2 macrophage polarization in PC. (a) The mRNA expression of M2 macrophage marker CD206 in PMA-pretreated THP-1 cells (M0), IL-4- and IL-13-treated M0 cells (M2), and M0 cells treated with PC cells with or without CXCL14 knockdown. (b) The mRNA expression of M2 macrophage-associated gene IL-10 in M0, M2, and M0 macrophage treated with PC cells with or without CXCL14 knockdown. (c) Immunofluorescence assay for the mRNA expression of M2 macrophage-associated gene IL-10 in M0, M2, and M0 macrophage treated with PC cells with or without CXCL14 knockdown. Scale bar: 25 μm. (d) The mRNA expression of M1 macrophage marker CD64 in PMA-pretreated THP-1 cells (M0), IFN-γ- and LPS-treated M0 cells (M1), and M0 cells treated with PC cells with or without CXCL14 knockdown. (e) The mRNA expression of M1 cytokines TNF-α in M0, M1, and M0 cells treated with PC cells with or without CXCL14 knockdown. , , and .

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