Exosomal CXCL14 Contributes to M2 Macrophage Polarization through NF-κB Signaling in Prostate Cancer

Figure 5

CXCL14 contributed to M2 macrophage polarization through NF-κB signaling. (a) The mRNA expressions of IκBα and NF-κB in PMA-pretreated THP-1 cells (M0) coculturing with LNCaP cells with or without CXCL14 knockdown. (b) The protein expression of NF-κB in M0 macrophages coculturing with LNCaP cells with or without CXCL14 knockdown. (c) The mRNA expressions of IL-10 and TNF-α in M0 macrophages treated with LNCaP cells without CXCL14 knockdown, LNCaP cells with CXCL14 knockdown, or LNCaP cells with CXCL14 knockdown plus NF-κB signaling activator betulinic acid (BA). (d) The mRNA expressions of IL-10 and TNF-α in M0 macrophages treated with LNCaP cells without CXCL14 knockdown, LNCaP cells with CXCL14 knockdown, or LNCaP cells with CXCL14 knockdown plus NF-κB signaling inhibitor JSH-23. (e) Wound healing assay for LNCaP cells treated with si-NC, si-CXCL14, or si-CXCL14 plus BA. Scale bar: 25 μm. (f) Transwell invasion assay for LNCaP and PC-3 cells treated with si-NC, si-CXCL14, or si-CXCL14 plus BA. , , and .

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