Ginkgolide C Alleviates Acute Lung Injury Caused by Paraquat Poisoning via Regulating the Nrf2 and NF-κB Signaling Pathways

Figure 7

Effects of GC on expressions of HO-1, NQO-1, GCLM, ICAM-1, VCAM-1, iNOS, IKK-β, p-IκB-α, Bcl-2, Bcl-xl, Bax, caspase-3, caspase-9, and nuclear translocations of Nrf2 and NF-κB p65 by Western blot in PQ-injured RLE-6TN cells. (a1) GC increased the expressions of HO-1, NQO-1, and GCLM after PQ treatment. (b) GC increased the translocation of Nrf2 from cytosolic (b1) to nuclear (b2). (c1) GC reduced the expressions of ICAM-1, VCAM-1, and iNOS. (d1) GC inhibited the expressions of IKK-β and p-IκB-α. (e) GC restrained the translocation of NF-κB p65 from cytosolic (e1) to nuclear (e2). (f1) GC increased the expressions of Bcl-2 and Bcl-xl and reduced the expression of Bax, caspase-3, and caspase-9. The levels of Nrf2 and NF-κB p65 were separately determined in cytosolic and nuclear extracts. Results were expressed as protein/reference protein ratio in each group (a2/b3/c2/d2/e3/f2). Data were expressed as of three independent experiments. ^## vs. the control group; vs. the PQ group.

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