Ginkgolide C Alleviates Acute Lung Injury Caused by Paraquat Poisoning via Regulating the Nrf2 and NF-κB Signaling Pathways

Figure 9

Effects of GC on expressions of HO-1, NQO-1, GCLM, ICAM-1, VCAM-1, iNOS, IKK-β, p-IκB-α, Bcl-2, Bcl-xl, Bax, caspase-3, and caspase-9 and nuclear translocations of Nrf2 and NF-κB p65 by Western blot in PQ-injured Nrf2 gene silencing RLE-6TN cells. GC had no effect on expressions of HO-1, NQO-1, and GCLM (a1); nuclear translocation of Nrf2 (b1/b2); expressions of ICAM-1, VCAM-1, and iNOS (c1); expressions of IKK-β and p-IκB-α (d1); nuclear translocation of NF-κB p65 (e1/e2); and expressions of Bcl-2, Bcl-xl, Bax, caspase-3, and caspase-9 (f1). The levels of Nrf2 and NF-κB p65 were separately determined in cytosolic and nuclear extracts. Results were expressed as protein/reference protein ratio in each group (a2/b3/c2/d2/e3/f2). Data were expressed as of three independent experiments. ^## vs. the control group.

(a)

(b)

(c)

(d)

(e)

(f)