Novel piRNA Regulates PIWIL1 to Modulate the Behavior of Placental Trophoblast Cells and Participates in Preeclampsia

Figure 7

(a–c) Overexpression of PIWIL1 promotes the proliferation and invasion and inhibits apoptosis of HTR-8/SVneo cells. (a) CCK-8 assay showing the relative proliferation rates of HTR-8/SVneo cells with and without PIWIL1 overexpression. (b) Representative images and quantitative analysis of a transwell assay show increased invasion of HTR-8/SVneo cells overexpressing PIWIL1. (c) Representative images and quantitative analysis of a flow cytometry assay showing apoptosis of HTR-8/SVneo cells overexpressing PIWIL1. (d–f) Silencing of PIWIL1 decreases the proliferation and invasion and increases apoptosis of HTR-8/SVneo cells. (d) CCK-8 assay showing the relative proliferation rate of HTR-8/SVneo cells after silencing PIWIL1 expression. (e) Representative images and quantitative analysis of a transwell assay showing the invasion of PIWIL1-silenced HTR-8/SVneo cells. (f) Representative images and quantitative analysis of a flow cytometry assay showing apoptosis of PIWIL1-silenced HTR-8/SVneo cells. compared with HTR-8/SVneo and HTR-8/SVneo+NC cells.

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