MANF Inhibits α-Synuclein Accumulation through Activation of Autophagic Pathways

Figure 1

The protective effect of AAV8-MANF on PD model and its role on the expression of lysosome-related genes in substantia nigra. (a) AAV8-MANF (with His tag) was injected into the substantia nigra of rat brain. The expression of MANF in DA neurons was detected by double immunofluorescence staining using anti-His tag antibody and anti-TH antibody. Scale bar is 200 μm. (b) The motor function of AAV8-MANF on rotenone-induced PD mouse models was evaluated by rotarod analysis. Ctrl group: ; rotenone group: ; : ; group: . (c, d) The effect of AAV8-MANF on rotenone-induced DA neuron degeneration was determined by TH staining. Scale bar is 500 μm. (e, f) The level of SNCA in substantia nigra was detected using western blot analysis. group and group: . (g, h) RNA-sequence analysis of gene expression in substantia nigra followed by AAV8-MANF injection. (e) Heatmap analysis. (f) KEGG pathway analysis. Data were expressed as from three independent experiments. ^# and ^## vs. Ctrl group; vs. -treated group.

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