MANF Inhibits α-Synuclein Accumulation through Activation of Autophagic Pathways

Figure 2

Effects of MANF on the degradation of SNCA^WT in PD cellular model. (a, b) SNCA^WT SH-SY5Y cells were treated with MANF (0, 250, 500, 1000, or 2000 ng/ml) and Dox (600 ng/ml) for 24 h, and cell lysates were immunoblotted by anti-SNCA antibody. (c, d) SNCA^WT SH-SY5Y cells were treated with MANF (500 ng/ml) and Dox (600 ng/ml) for 24 h, and the level of SNCA^WT was detected by immunofluorescence staining. Scale bar is 50 μm. (e)–(g) SNCA^WT H-SY5Y cells were treated with MANF (500 ng/ml) and Dox (600 ng/ml) for 24 h, followed by RNA-sequence analysis. (e) Heatmap analysis. (f) KEGG pathway analysis. (g) Target genes involved in lysosomal-related degradation pathway. Data were expressed as from three independent experiments. ^### vs. control group; and vs. Dox-treated group.

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