Research Article
MANF Inhibits α-Synuclein Accumulation through Activation of Autophagic Pathways
Figure 6
MANF inhibited the accumulation of SNCAA53T in PD cellular model. (a, b) SNCAA53T SH-SY5Y cells were treated with MANF (0, 250, 500, 1000, or 2000 ng/ml) and Dox (600 ng/ml) for 24 h, and cell lysates were immunoblotted by anti-SNCA antibody. (c, d) SNCAA53T SH-SY5Y cells were treated with MANF (500 ng/ml) and Dox (600 ng/ml) for 24 h, and the level of SNCA was detected by immunofluorescence staining. Scale bar is 50 μm. (e)–(h) Effects of MANF on the levels of SNCA, Lamp-2A, and Hsc70. SNCAA53T SH-SY5Y cells were treated with MANF (250 and 500 ng/ml) and Dox (600 ng/ml) for 24 h (a, b) or 48 h (c, d), respectively; then, the protein levels were detected by western blot analysis. Data were expressed as from three independent experiments. ## vs. control group; , vs. Dox-treated group.
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