Urocortin I Protects against Myocardial Ischemia/Reperfusion Injury by Sustaining Respiratory Function and Cardiolipin Content via Mitochondrial ATP-Sensitive Potassium Channel Opening

Figure 2

Perfusion protocol. (a) N₁ group was perfused with 37°C K-H solution continuously for 155 min. IR group was perfused with 4°C St. Thomas cardioplegia after 20 min 37°C K-H solution perfusion and was kept ischemia at 32°C for 40 min and then reperfused with 37°C K-H solution for 95 min. U₁ group was reperfused with 37°C K-H solution that contained urocortin I for 30 min after ischemia and then reperfused with 37°C K-H solution for 65 min. HU₁ group was reperfused with 37°C K-H solution that contained 5-HD for 5 min before urocortin I administration, and the rest procedures were the same as U₁ group. (b) N₂ group was continuously cultured in the 37°C-incubator for 150 min. HR group was reoxygenated for 110 min after 40 min hypoxia. U₂ group was reoxygenated for 10 min after 40 min hypoxia then cultured in the medium containing urocortin I for 30 min and reoxygenated for 70 min. HU₂ group was cultured in the medium that contained 5-HD for 10 min after 40 min hypoxia, and the rest procedures were the same as the U₂ group.

(a)

(b)