N-((1-(4-Fluorophenyl)-1H-1,2,3-triazol-4-yl)methyl)-2-methylene-3-oxo-olean-12-en-28-amide Induces Apoptosis in Human Breast Cancer Cells by Stimulating Oxidative Stress and Inhibiting the Notch-Akt Signaling Pathway

<div>ZQL-4c induces apoptosis in breast cancer cells. MCF-7, MDA-MB-231, and SK-BR-3 cells were treated with 0, 0.4, 0.8, and 1.6 <i>μ</i>mol/L ZQL-4c for 24 h. (a) Light microscopy showed apoptosis-related changes induced by ZQL-4c. (b) Cells were stained with annexin V/PI and analyzed by flow cytometry. (c) The percentage of apoptotic cells is shown in the histogram. 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(d) The protein levels of cleaved caspase-3,-7,-9, p53, Bax, and Bcl-2 were detected by western blotting. Actin was used as a loading control.</div>

Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: N-((1-(4-Fluorophenyl)-1H-1,2,3-triazol-4-yl)methyl)-2-methylene-3-oxo-olean-12-en-28-amide Induces Apoptosis in Human Breast Cancer Cells by Stimulating Oxidative Stress and Inhibiting the Notch-Akt Signaling Pathway 

Figure 4 | N-((1-(4-Fluorophenyl)-1H-1,2,3-triazol-4-yl)methyl)-2-methylene-3-oxo-olean-12-en-28-amide Induces Apoptosis in Human Breast Cancer Cells by Stimulating Oxidative Stress and Inhibiting the Notch-Akt Signaling Pathway 