HNF4A Regulates the Proliferation and Tumor Formation of Cervical Cancer Cells through the Wnt/β-Catenin Pathway

Figure 1

The expression of HNF4A is downregulated in cervical cancer. (a) Immunohistochemical staining of HNF4A in clinical samples, including the normal cervix (NC, ) and cervical carcinomas (CC, ) (original magnification, 1000x). (b) The immunohistochemical staining intensity was classified into negative, weak positive, and strong positive, and the percentage of each group was shown. (c) The scatter plots showed the IHC scores obtained for the staining of HNF4A in different cervix lesion samples (points represent the IHC score per specimen, and Student’s -test is performed). (d) HNF4A expression was detected by western blot in 8 normal cervix samples and 8 cervical carcinoma samples. GAPDH was used as a loading control. (e) The quantitative illustration of the levels of HNF4A protein using densitometry to measure the density of the corresponding bands in (d). Student’s -test was carried out. (f) The relationship between relapse-free survival (RFS) probability of CESC patients () and the expression level of HNF4A in their tumors was shown by the Kaplan–Meier estimator in TCGA database. , .

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