HNF4A Regulates the Proliferation and Tumor Formation of Cervical Cancer Cells through the Wnt/β-Catenin Pathway

Figure 5

HNF4A downregulated the activity of the Wnt/β-catenin pathway. (a) Heatmap of the data from RNA-seq. (b) The result of gene set enrichment analysis. (c) The significantly changed genes in GSEA. (d, e) TOP/FOP-Flash reporter assays were carried out in HNF4A-modified cervical cancer cells. (f, g) Real-time PCR analysis is shown for the mRNA levels of the Wnt/β-catenin pathway key genes in HNF4A-modified cervical cancer cells. (h, i) The expression of Wnt/β-catenin pathway key proteins in HNF4A-modified cervical cancer cells was determined by western blotting. (j, k) The quantitative analysis of the western blotting in (h) and (i). Data represent of triplicate experiments, and statistical analysis was done by Student’s -test. , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)