Apigenin Induced Apoptosis by Downregulating Sulfiredoxin Expression in Cutaneous Squamous Cell Carcinoma

Figure 1

Apigenin can downregulate Srx expression in TPA-induced JB6 and A431 cSCC cells. (a) JB6 was treated with control (DMSO), TPA (20 nM), or apigenin (40 or 80 μM) for different time points (6 h-48 h). Srx expression was detected by western blotting (left). Western blot was served to analyze the expression of Srx while TPA-induced JB6 cells were incubated with apigenin (80 μM) for different times (6 h-48 h) (right). (b) Human SCC A431 cells were treated with apigenin at different times, and the expression of Srx was detected by WB. (c) The mRNA level of Srx in TPA-induced JB6 and A431 cells was conducted to measure after incubation with 80 μM apigenin for 6 h and 12 h. (d) Representative images of immunofluorescence staining of Srx in TPA-induced JB6 treated with control (DMSO) or 80 μM apigenin for 6 h-48 h (left). Quantitative analysis of Srx means fluorescence intensity (MFI) (right) (, ). Significant differences were evaluated using a one-way ANOVA. , , and vs. control. For JB6 cells, TPA-induced sample as control.

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