Apigenin Induced Apoptosis by Downregulating Sulfiredoxin Expression in Cutaneous Squamous Cell Carcinoma

Figure 4

Apigenin activated the MAPK signaling pathway in cSCC in vitro. (a, b) Variation of the MAPK signaling pathway with the treatment of apigenin in TPA-induced JB6 (a) and A431 cells (b). The cells were treated with 80 μM apigenin for different time points up to 24 h. Western blot was applied to analyze the expression of MAPK pathway-associated proteins, includingp38, ERK1/2 and JNK compared with GAPDH (left). The bar graph on the right showed the intensity of the phosphorylation protein band from each treatment relative to the total protein. Valued represent the . Significant difference was designed by ANOVA, , , , and vs. control (TPA-induced sample as control for JB6 cells). (c) TPA-induced JB6 cell was incubated with control (DMSO, TPA-alone, and apigenin-alone) or apigenin (40 or 80 μM) for indicted time points. Western blot was served to analyze the expression of Nrf2. β-Actin was used as the reference for the loading quantity of protein sample. The bar graph indicated the density quantification of the Nrf2 band relative to β-actin. and vs. TPA-induced control by ANOVA.

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