Intrinsic ROS Drive Hair Follicle Cycle Progression by Modulating DNA Damage and Repair and Subsequently Hair Follicle Apoptosis and Macrophage Polarization

Figure 10

Cas 3 inhibition enhanced the capacity of DNA repair. (a–m) Western blot showed that expression of γH2AX was upregulated in the hair follicle in mice treated with 4 and 8 mg/kg Ac. Expression of 116 kDa PARP1 and PAR was enhanced in mice treated with Ac as indicated. Expression of DNA damage repair proteins, p-ATM, p-BRCA 1, Rad51, KU80, and KU70, was increased in mice treated with Ac at 4 and 8 mg/kg. Translocation of AIF to the nucleus was enhanced in the hair follicle, while c-Cas 3 was downregulated in mice treated with Ac. , , and .

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