Intrinsic ROS Drive Hair Follicle Cycle Progression by Modulating DNA Damage and Repair and Subsequently Hair Follicle Apoptosis and Macrophage Polarization

Figure 15

Cas 3 inhibition promoted double-strand break (DSB) repair in HF cells, and PARP1 inhibition promoted activation of the Cyto c/Cas 3 pathway. (a–m) The results of western blot showed that the marker of DNA break γH2AX was upregulated in mice treated with inhibitors. Expression of 116 kDa PARP1 and PAR in the hair follicle was increased in mice treated with Ac, followed by the DNA repair proteins, while AIF translocation was enhanced and activation of Cas 3 was weakened. Expression of PAR was decreased in mice treated with BMN 673, followed by the DNA repair proteins, while expression of Cyto c and activation of Cas 3 were enhanced and AIF translocation was weakened. There was no significant change in the expressions of DNA repair proteins, except an increase in γH2AX and Cyto c and decrease in KU80 in mice treated with both Ac and BMN 673 (Ac+B). (n, o) More NAD+ and NADH were consumed in hair follicles in mice treated with Ac, in contrast to the mice treated with BMN 673. , , , and .

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