Intrinsic ROS Drive Hair Follicle Cycle Progression by Modulating DNA Damage and Repair and Subsequently Hair Follicle Apoptosis and Macrophage Polarization

Figure 2

Nuclear translocation of AIF and activation of caspase-3 were consistent with the cell death in HFs. (a, b) 57 kDa AIF translocated into the nucleus in TUNEL-positive cells, and MFI of 57 kDa AIF was enhanced in catagen consistent with TUNEL. (c, d) Cleaved caspase-3 (c-Cas 3) was activated in TUNEL-positive cells during the hair cycle, while MFI of c-Cas 3 was decreased in early catagen and elevated in late catagen. The insert boxes in the figure are partially enlarged pictures. Horizontal line in the statistical analysis graph means that each of the groups under the line was statistically different compared with another group. , , and . Scale bar (a, c): 200 μm.

(a)

(b)

(c)

(d)