Pomelo Peel Essential Oil Ameliorates Cerebral Ischemia-Reperfusion Injury through Regulating Redox Homeostasis in Rats and SH-SY5Y Cells

Figure 3

(a) Immunohistochemistry staining for SLC7A11 and GPX4 in cortex neurons (400x) and statistical analysis bar graph in each group, respectively. SLC7A11 or GPX4-positive neuron was stained brown. The data were expressed as the ( per group). ^# and ^## compared to the sham group; and compared to the NS group. (b and d) Western blot analysis of GPX4 and SLC7A11 protein expression in vivo and in vitro. The data were expressed as the ( per group). ^# compared to the sham group; compared to the NS group. (c) Measurement of the level of GSH in different groups or SH-SY5Y cells. ^# compared to the sham group and or P<0.01 compared to the NS group. (e) GSH content assay in SH-SY5Y cells. ^# or ^## compared to the control group and P<0.01 compared to the OGD+PPEO group.

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