Interleukin-22 Ameliorates Dextran Sulfate Sodium-Induced Colitis through the Upregulation of lncRNA-UCL to Accelerate Claudin-1 Expression via Sequestering miR-568 in Mice

Figure 6

Claudin-1 is the direct target of miR-568 in intestinal epithelial cells. Sequence alignment showing the binding sites between miR-568 and the 3-UTR of claudin-1 mRNA. (b) Western blot analysis was used to detect changes in claudin-1 protein expression under the effect of miR-568 mimics and inhibitor in MODE-K cells. (c) Luciferase assay of HEK-293T cells cotransfected with miR-568 mimics and wild-type or mutant 3-UTR of the claudin-1 mRNA sequence. (d–f) qRT-PCR, western blotting, and immunohistochemistry analyses were conducted to determine claudin-1 expression levels in the colon tissues of all groups of mice. Data are presented as . ^##; ^# using Student’s -test.

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