lncRNA SNHG15 Induced by SOX12 Promotes the Tumorigenic Properties and Chemoresistance in Cervical Cancer via the miR-4735-3p/HIF1a Pathway

Figure 3

lncRNA SNHG15 expression in cervical cancer is transcriptionally regulated by SOX12. The upstream regulator of lncRNA SNHG15, predicted using the JASPAR (http://jaspar.genereg.net/) database. (a, b) SiHa and HeLa cells stably transfected with siRNAs; lncRNA SNHG15 expression, measured by qRT-PCR. (c) SiHa and HeLa cells transfected with pcDNA3.1-SOX12 and its normal control; lncRNA SNHG15 expression was assessed by qRT-PCR. (d) Binding motif of SOX12 predicted by JASPAR. (e) Putative binding sites for the lncRNA SNHG15 promoter, determined using JASPAR. (f) Binding possibility between SOX12 and the lncRNA SNHG15 promoter, assessed by ChIP assay. (g, h) Interaction between SOX12 and the lncRNA SNHG15 promoter in (g) SiHa and (h) HeLa cells, verified using the luciferase reporter gene assay. Data are presented as ; ; .

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