lncRNA SNHG15 Induced by SOX12 Promotes the Tumorigenic Properties and Chemoresistance in Cervical Cancer via the miR-4735-3p/HIF1a Pathway

Figure 4

lncRNA SNHG15 sponges miR-4735-3p. Putative downstream targets for lncRNA SNHG15, predicted using the starBase (http://starbase.sysu.edu.cn/) dataset (CLIP Data: strict stringency (≥5)). (a, b) Potential miRNA targets for lncRNA SNHG15 in SiHa and HeLa cells, evaluated by biotinylated RNA pull-down; expression levels of putative miRNA targets in biotinylated probe bounds were measured by qRT-PCR. (c) Expression levels of lncRNA SNHG15 in Bio-miR-4735-3p-WT or Bio-miR-4735-3p-Mut bounds, measured by qRT-PCR. (d) Binding sites between lncRNA SNHG15 and miR-4735-3p, obtained using the starBase dataset. (e) Transfection efficiency of the miR-4735-3p mimic, evaluated by qRT-PCR. (f, g) Association between lncRNA SNHG15 and miR-4735-3p, assessed using the luciferase reporter gene assay in (f) SiHa and (g) HeLa. (h) miR-4735-3p expression in lncRNA SNHG15 knockdown cervical cancer (CC) cells, assessed by qRT-PCR. (i) miR-4735-3p expression in CC tumor samples () and adjacent normal samples (), measured by qRT-PCR. Data are presented as ; ; ; .

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