lncRNA SNHG15 Induced by SOX12 Promotes the Tumorigenic Properties and Chemoresistance in Cervical Cancer via the miR-4735-3p/HIF1a Pathway

Figure 6

miR-4735-3p targets to HIF1a. (a) Putative downstream targets for miR-4735-3p, predicted by microT (http://www.microrna.gr/microT), miRmap (http://mirnamap.mbc.nctu.edu.tw/), PicTar (http://www.pictar.org/), and TargetScan (http://www.targetscan.org/) with CLIP Data: strict stringency (≥5) and . (b, c) Putative mRNA targets in (b) SiHa and (c) HeLa cells upon miR-4735-3p overexpression were measured by qRT-PCR. (d) HIF1a expression in cervical cancer (CC) cells upon miR-4735-3p downregulation, measured by qRT-PCR. (e) Binding sites between HIF1a and miR-4735-3p. (f, g) Association between miR-4735-3p and HIF1a in (f) SiHa and (g) HeLa cells, evaluated by ChIP assay; analysis of results was by qRT-PCR. (h, i) Interaction between HIF1a and miR-4735-3p, assessed using the luciferase reporter gene assay in (h) SiHa and (i) HeLa. (j) HIF1a expression in CC tumor samples () and adjacent normal samples (), measured by qRT-PCR. (k) HIF1a expression in 4 randomly chosen pairs of CC tissues, detected by Western blot. Data are presented as ; ; .

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