Circ_000829 Plays an Anticancer Role in Renal Cell Carcinoma by Suppressing SRSF1-Mediated Alternative Splicing of SLC39A14

Figure 1

Circ_000829 is underexpressed in RCC, and its upregulation inhibited RCC cell proliferation and induced cell apoptosis. (a) Venn diagram presenting the circRNAs that may interact with SRSF1 in RCC samples predicted by intersection of the Circinteractome website and the GSE100186 dataset. (b) The hsa_circ_000829 expression in RCC samples in the GSE100186 dataset ( for each dataset). (c) RT-qPCR analysis of the expression of circ_000829 in clinical RCC tissues and adjacent normal tissues. (d) Northern blot analysis of circ_000829 expression in clinical RCC tissues and adjacent normal tissues (). (e) RT-qPCR analysis of circ_000829 overexpression efficiency in A498 and 786-O cells. (f) Cell cycle distribution detected by flow cytometry in the presence of overexpression of circ_000829. (g) Cell proliferation assessed by EdU assay in the presence of overexpression of circ_000829. (h) RT-qPCR analysis of knockdown efficiency of sh-circ_000829 in A498 and 786-O cells. (i) Knockdown efficiency of sh-circ_000829 in A498 and 786-O cells confirmed by northern blot analysis. (j) Cell cycle distribution detected by flow cytometry in the presence of sh-circ_000829. (k) Cell proliferation assessed by EdU assay in the presence of sh-circ_000829. (l) Flow cytometric analysis of apoptosis of A498 and 786-O cells in the presence of circ_000829 vector or sh-circ_000829. . The cell experiments were conducted three times independently.

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