Circ_000829 Plays an Anticancer Role in Renal Cell Carcinoma by Suppressing SRSF1-Mediated Alternative Splicing of SLC39A14

Figure 2

Circ_000829 binds to SRSF1 and inhibits its expression. (a) The binding of SRSF1 antibody to circ_000829 assessed by the RIP assay. (b) The binding of SRSF1 to biotin-circ_000829 in the RNA pull down assay. (c) RT-qPCR analysis of SRSF1 expression in 67 pairs of RCC and adjacent normal tissues. (d) IHC analysis of SRSF1 protein in 67 pairs of RCC and adjacent normal tissues. Blue indicates the nucleus, and brown indicates SRSF1 positive cells. (e) Pearson analysis of the correlation between the relative expression of circ_000829 and SRSF1 in RCC tissues (). (f) Western blot of SRSF1 expression in A498 and 786-O cells in the presence of sh-circ_000829. (g) Western blot of SRSF1 protein expression in A498 and 786-O cells in the presence of overexpression of circ_000829. . The cell experiments were conducted three times independently.

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