Circ_000829 Plays an Anticancer Role in Renal Cell Carcinoma by Suppressing SRSF1-Mediated Alternative Splicing of SLC39A14

Figure 3

Circ_000829 targets SRSF1 to repress the proliferation of RCC cells and induce their apoptosis in vitro. (a) Western blot of the SRSF1 expression in A498 and 786-O cells transduced with circ_000829 vector alone or combined with SRSF1 vector. (b) Proliferation of A498 and 786-O cells transduced with circ_000829 vector alone or combined with SRSF1 vector, as assessed by EdU assay. (c) The cell cycle of A498 cells transduced with circ_000829 vector alone or combined with SRSF1 vector detected by flow cytometry. (d) The cell cycle of 786-O cells transduced with circ_000829 vector alone or combined with SRSF1 vector detected by flow cytometry. (e) Flow cytometric analysis of apoptosis of A498 and 786-O cells transduced with circ_000829 vector alone or combined with SRSF1 vector. . n.s indicates no statistical significance. The cell experiments were conducted three times independently.

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