Circ_000829 Plays an Anticancer Role in Renal Cell Carcinoma by Suppressing SRSF1-Mediated Alternative Splicing of SLC39A14

Figure 4

SRSF1 induces alternative splicing of SLC39A14 mRNA. (a) Knockdown efficiency of sh-SRSF1 in A498 and 786-O cells confirmed by western blot. (b) RT-qPCR analysis of the effect of sh-SRSF1 on the SLC39A14A and SLC39A14B expression. (c) RT-qPCR analysis of the effect of SRSF1 overexpression on the SLC39A14A and SLC39A14B expression. (d) SLC39A14A and SLC39A14B expression in A498 and 786-O cells following SRSF1 silencing determined by northern blot analysis. (e) SLC39A14A and SLC39A14B expressions in A498 and 786-O cells following SRSF1 overexpression determined by northern blot analysis. (f) The role of FLAG-SRSF1 in the SLC39A14A/B expression in A498 and 786-O cells assessed by RIP assay. (g) RT-qPCR analysis of SLC39A14 pre-mRNA following SRSF1 overexpression or knockdown. . n.s indicates no statistical significance. The cell experiments were conducted three times independently.

(a)

(b)

(c)

(d)

(e)

(f)

(g)