Circ_000829 Plays an Anticancer Role in Renal Cell Carcinoma by Suppressing SRSF1-Mediated Alternative Splicing of SLC39A14

Figure 5

SLC39A14B abolishes the antiproliferation and proapoptosis effects of SLC39A14 knockdown on RCC cells. (a) Analysis of SLC39A14 protein expression in RCC based on GEPIA database. (b) RT-qPCR analysis of the expression of SLC39A14B in RCC tissues and adjacent normal tissues (). (c) Northern blot analysis of SLC39A14B expression in RCC tissues and adjacent normal tissues (). (d) RT-qPCR analysis of SLC39A14A and SLC39A14B expression in A498 and 786-O cells transduced with sh-SLC39A14 alone or combined with SLC39A14A vector or SLC39A14B vector. (e) The proliferation of A498 and 786-O cells transduced with sh-SLC39A14 alone or combined with SLC39A14A vector or SLC39A14B vector detected by EdU. (f) Flow cytometry detection of the cell cycle of A498 and 786-O cells transduced with sh-SLC39A14 alone or combined with SLC39A14A vector or SLC39A14B vector. (g) Flow cytometric analysis of apoptosis of A498 and 786-O cells transduced with sh-SLC39A14 alone or combined with SLC39A14A vector or SLC39A14B vector. . n.s means no statistical significance. The cell experiments were conducted three times independently.

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