Research Article

Early Development of Cardiac Fibrosis in Young Old-Father Offspring

Figure 2

Cardiac remodeling in hypertensive old father offspring. (a) Perivascular coronary, epicardial, and myocardial histological sections stained with Masson’s trichrome were obtained from different animal groups. The green stains represent the fibrotic areas. (b) Representative images of immunofluorescence staining (594 nm) for wheat germ agglutinin (WGA) in the hearts of different animal groups. (c) Quantifications of interstitial reactive, perivascular, replacement, and epicardial fibrosis, as well quantifications of immunofluorescence staining for WGA. Scale bars: 100 μm in (a) and 50 μm in (b). Magnifications: ×100 in (a) and ×200 in (b). , , , and .
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