Early Development of Cardiac Fibrosis in Young Old-Father Offspring

Figure 3

Cardiac remodeling in hypertensive old father offspring. (a) Immunofluorescence staining (594 nm) for α-SMA of histological sections obtained from the hearts of different animal groups, as well as quantifications of the respective stained zones. White asterisks indicate coronary vessels. (b–e) Immunofluorescence staining (488 nm) for PDGFRα, Tcf21, Tbx18, and Wt1 of histological sections obtained from the hearts of different animal groups, as well as quantifications of the respective stained zones. The insets in all of the panels represent a higher magnification to highlight the interstitial fibroblast distribution. Scale bars: 50 μm. Magnifications: ×200. , , , and .

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