BMSC-EV-derived lncRNA NORAD Facilitates Migration, Invasion, and Angiogenesis in Osteosarcoma Cells by Regulating CREBBP via Delivery of miR-877-3p

Figure 1

Isolation and identification of BMSCs and BMSC-EVs. (a) BMSC (P3) morphology observed under a microscope; (b) osteogenic, adipogenic, and chondrogenic differentiation of BMSCs detected by Alizarin Red, Oil Red O, and Alcian Blue staining; (c) expressions of BMSC surface markers CD29, CD90, CD34, and CD45 measured by flow cytometry; (d) BMSC-EV morphology observed under a TEM; (e) size distribution of BMSC-EVs measured by the qNano system; (f) expressions of BMSC-EV-specific markers CD63, CD81, TSG101, and Calnexin measured by Western blot. The BMSC culture supernatant after GW4869 intervention served as the negative control (GW).

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