BMSC-EV-derived lncRNA NORAD Facilitates Migration, Invasion, and Angiogenesis in Osteosarcoma Cells by Regulating CREBBP via Delivery of miR-877-3p

Figure 4

NORAD carried by BMSC-EVs upregulated CREBBP by sponging miR-877-3p. (a) Potential binding site of NORAD and miR-877-3p predicted on the LncBase Predicted v.2; (b) potential binding site of miR-877-3p and CREBBP predicted on the TarBase v.8; (c) binding relationship of NORAD and miR-877-3p validated by the dual-luciferase assay; (d) binding relationship of miR-877-3p and CREBBP validated by the dual-luciferase assay; (e) binding relationship of NORAD and miR-877-3p verified by the RIP assay; (f) expressions of miR-877-3p and CREBBP in hFOB 1.19, 143B, MG63, Saos2, HOS, and U-2OS cells detected by RT-qPCR; (g) expressions of miR-877-3p and CREBBP in OS cells detected by RT-qPCR; (h, i) expression of CREBBP in OS cells detected by Western blot. Cell experiment was repeated 3 times. Data were presented in the form of . The independent-sample test was used for pairwise comparisons, one-way ANOVA for comparisons among multigroups, and Tukey’s multiple comparisons test was used for a post hoc test. , .

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