Methylglyoxal Scavengers Attenuate Angiogenesis Dysfunction Induced by Methylglyoxal and Oxygen-Glucose Deprivation

Figure 7

The chick embryo chorioallantoic membrane (CAM) assay was designed to study the antiangiogenesis capacity of MG. (a) A diagrammatic sketch depicting how and when to inject the drugs and how embryos were harvested from the eggs. (b–e, b1–e1) Representative bright-field images of chicken embryo CAM vascular plexus were taken from the control, 0.5 mmol/L MG, 1 mmol/L MG, and 2 mmol/L MG groups. (b2–e2) The representative H&E stained cross sections of the parts have been indicated by the lines in (b1)–(e1), respectively. DAPI (b3–e3), Cav1 (b4–e4), and merged (b5–e5) immunofluorescence staining shows the cross section of the part indicated by the lines in (b1)–(e1). (f) Statistical analysis of chicken embryo mortality. (g) The vascular plexus densities on chick embryo CAM. (h) Comparison of chick embryo CAM vessel diameter. All the data shown are the (), , , and vs. the control group.  mm in (b)–(e); 2 mm in (b1)–(e1); 100 μm in (b2)–(e5).