Novel Diagnostic Biomarkers Related to Oxidative Stress and Macrophage Ferroptosis in Atherosclerosis

<div>In vitro and in vivo experiments verified the expression of hub genes. (a) Relative mRNA level of <i>ALOX5</i>, <i>NCF1</i>, <i>NCF2</i>, and <i>NCF4</i> in RAW264.7. (b) HE stains showed control group in C57BL/6 mice and AS group in APOE-/- mice. (c and d) Immunohistochemistry staining of <i>ALOX5</i> and <i>NCF2</i> in the aortic valve. Analysis of <i>ALOX5</i>-positive area and <i>NCF2</i>-positive area in immunohistochemistry stains. (e, g) Increased <i>ALOX5</i> and <i>NCF2</i> expression in AS tissues and control tissues were detected by western blotting. 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Oxidative Medicine and Cellular Longevity

fig14

Figure 14

Figure 14: Novel Diagnostic Biomarkers Related to Oxidative Stress and Macrophage Ferroptosis in Atherosclerosis 