Research Article

Cell-Dependent Pathogenic Roles of Filamin B in Different Skeletal Malformations

Figure 6

Expression patterns of wild-type and FLNB mutants and osteogenesis-related marker Runx2 as well as upstream signaling pathways in Saos-2 cells. Whole Saos-2 cell lysates from transiently transfected with WT or mutant FLNB plasmids were fractioned on 10% SDS-PAGE and analyzed by immunoblotting (). Quantitative analysis of FLNB, SHIP2, p-PI3K, t-PI3K, p-AKT, t-AKT, p-SMAD3, t-SMAD3, and Runx2 levels was shown by the normalized to GAPDH levels (, , and by Student’s -test).