α-Lipoic Acid-Plus Ameliorates Endothelial Injury by Inhibiting the Apoptosis Pathway Mediated by Intralysosomal Cathepsins in an In Vivo and In Vitro Endothelial Injury Model

Figure 2

Time course of the protein levels of cathepsin B/D after CAII. (a, b) Western blot revealed the protein levels of cathepsin B/D at sham; 6, 12, 24, 48, and 72 h; and 1 week, 2 weeks, and 4 weeks after CAII. (c, d) Quantification of cathepsin B/D protein levels was normalized to the sham group. (e, g) IF analysis was performed with antibodies against cathepsin B/D (red) in CAII sections. Nuclei were labeled with DAPI (blue). Representative images of the sham group and CAII (48 h) group are shown. Scale bar, 100 μm. (f, h) Immunopositivity of cathepsins B and D in the endothelium. The sham group was used as the standard. Data are shown as the . relative to the sham group.

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