α-Lipoic Acid-Plus Ameliorates Endothelial Injury by Inhibiting the Apoptosis Pathway Mediated by Intralysosomal Cathepsins in an In Vivo and In Vitro Endothelial Injury Model

Figure 8

Changes in ferritin and TfR after DFO and LAP treatments under endothelial and HUVEC injury conditions. (a) Western blotting indicating ferritin and TfR expression levels in the endothelium. (b) Western blotting showing Ferritin and TfR expression levels in HUVECs. (c–f) The mean values of Ferritin and TfR protein levels in the sham and control groups were normalized to 1.0. (g, i) IF analysis was performed using Ferritin and TfR antibodies (green), and nuclei were fluorescently labeled with DAPI (blue). (h, j) The mean value of the fluorescence intensity of the control group was normalized to 1.0. (k) Iron deposition in the HUVECs was exhibited in different groups. Representative images are indicated. Scale bar, 100 μm. Data are shown as the . vs. sham group and control group; vs. CAII+vehicle group and OxyHb group; vs. CAII+DFO group and OxyHb+DFO group; .

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