HBO Alleviates Neural Stem Cell Pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 Axis and Improves Neurogenesis after Oxygen Glucose Deprivation

Figure 4

lncRNA-H19 functioned as a molecular sponge of miR-423-5p to target NLRP3. (a) The binding site between miR-423-5p and wild-type lncRNA-H19 (H19-WT) predicted by bioinformational tool LncBase V2; the mutant lncRNA-H19 (H19-MUT) sequence was shown as well. (b) Double luciferase assay was performed to verify the binding of lncRNA-H19 and miR-423-5p. vs. the negative control group (miR-NC). (c) Expression of miR-423-5p in NSCs transfected with either siRNA-H19 or pcDNA-H19 or corresponding negative control (siRNA-NC or pcDNA-NC) was examined by qPCR. vs. the Con group; ^# vs. the siRNA-NC group; ^† vs. the pcDNA-NC group. (d) The predicted binding site between miR-423-5p and the 3UTR of wild-type NLRP3 (NLRP3-WT) obtained from bioinformational tool microT-CDS; the mutant sequence of NLRP3 (NLRP3-MUT) was also shown. (e) The binding of miR-423-5p and NLRP3 was examined by double luciferase assay. vs. the miR-NC group. (f) Expression of NLRP3 mRNA in NSCs transfected with either miR-423-5p inhibitor or miR-423-5p mimic or corresponding negative control (miR-NC inhibitor or miR-NC mimic) was detected by qPCR. vs. the Con group; ^# vs. the miR-NC inhibitor group; ^† vs. the miR-NC mimic group. (g, h) WB determination and statistical analysis of NLRP3 protein in NSCs transfected as above. vs. the Con group; ^# vs. the miR-NC inhibitor group; ^† vs. the miR-NC mimic group.

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