HBO Alleviates Neural Stem Cell Pyroptosis via lncRNA-H19/miR-423-5p/NLRP3 Axis and Improves Neurogenesis after Oxygen Glucose Deprivation

Figure 6

HBO attenuated NSC pyroptosis by inhibiting lncRNA-H19/miR-423-5p/NLRP3 axis following OGD. (a) NSCs transfected with either pcDNA-NC or pcDNA-H19 or pcDNA-H19+miR-NC mimic or pcDNA-H19+miR-423-5p mimic were exposed to OGD and treated by HBO; the level of NLRP3 mRNA was analyzed with qPCR. (b) Expression of NLRP3, Caspase-1 p20, and GSDMD-N protein in NSCs treated as above was determined by WB. (c–e) Statistical analysis on the protein expression of NLRP3, Caspase-1 p20, and GSDMD-N in the above six groups. (f, g) The level of IL-1β and IL-18 released from NSCs in the above six groups was examined using ELISA. vs. the OGD group; ^♦ vs. the OGD+HBO group; ^# vs. the OGD+HBO+pcDNA-NC group; ^∆ vs. the OGD+HBO+pcDNA-H19 group; ^† vs. the OGD+HBO+pcDNA-H19+miR-NC mimic group.

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