IL-6 Deficiency Attenuates Skeletal Muscle Atrophy by Inhibiting Mitochondrial ROS Production through the Upregulation of PGC-1α in Septic Mice

Figure 4

The knockdown of PGC-1ɑ abolished the protective effects of IL-6 KO in CLP-induced skeletal muscle atrophy. RT-PCR (a) and western-blots (b) were performed to determine the mRNA and protein expressions of PGC-1ɑ in skeletal muscle of septic mice after PGC-1ɑ knockdown; (c) the immunoblots for the protein expressions of atrophy-related genes Atrogin-1 and MuRF-1; (d) mitochondrial ROS production was measured after PGC-1ɑ knockdown in septic mice; the cross-sectional area (CSA) of myofibers (e) and the maximum tetanic forces (f) were measured to evaluate muscle atrophy and contractile capacity. CLP: cecal ligation and puncture; CSA: cross-sectional area. , , and (ANOVA followed by unpaired Student -test, ).

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