HSPB8 Overexpression Ameliorates Cognitive Impairment in Diabetic Mice via Inhibiting NLRP3 Inflammation Activation

Figure 4

HSPB8 overexpression in the hippocampus alleviates mitochondrial fission. (a) Expressions of p-Drp1, Drp1, MFN1, MFN2, and OPA1 in the hippocampus were detected by Western blot. β-Actin was used as loading control. ( each group) and (b) quantitative analysis. , , , , , . (c, d) Representative images of immunofluorescence staining against p-Drp1 (green) in the (dentate gyrus) DG and CA1. Nuclei were counterstained with DAPI (blue) ( mice per group). . (e) Representative electron microscopy images showing mitochondrial morphology in hippocampal neurons ( mice per group). . (f, g) Measurements of mitochondrial numbers and mitochondrial length. One-way ANOVA test followed by Tukey’s post hoc test. Data are shown as the . , , .

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