HSPB8 Overexpression Ameliorates Cognitive Impairment in Diabetic Mice via Inhibiting NLRP3 Inflammation Activation

Figure 7

Overexpression of HSPB8 attenuates MMP and NLRP3 inflammasome activation in high glucose treated N2a cells. (a) Representative western blot images and quantitative analyses of HSPB8 in cells from all groups. β-Actin was used as loading control. (b) Representative images of JC-1 immunochemistry staining showing the mitochondrial membrane potential in each group of cells. (c) Relative quantitative comparison of red-to-green fluorescence intensity ratios between each group. (d–i) Expressions of p-Drp1, Drp1, NLRP3, cleaved caspase1 p20, and IL-1β in N2A cells of all groups were detected by western blot and quantitative analysis. β-Actin was used as loading control. All data are shown as the . Two-tailed Student’s -test, , , , , , , .

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