[Retracted] DNA Methyltransferase Inhibitor 5-AZA-DC Regulates TGFβ1-Mediated Alteration of Neuroglial Cell Functions after Oxidative Stress

Figure 2

5-AZA-DC intervention affected the binding between TGFβ1 and PTEN gene. (a) The interactions between TGFβ1 and PTEN (or TSC2) proteins were determined by GST-pull down assay. (b) CHIP was utilized to further verify the direct binding effect between TGFβ1 and PTEN gene. (c) Cells were subjected to oxyglucose deprivation/reoxygenic stimulation. The interaction between TGFβ1 and PTEN gene was detected using CHIP assay after TGFβ1^OE (or shTGFβ1) transfection and/or 5-AZA-DC treatment. (d) Dual-luciferase reporter assay was used to verify the binding between transcription factor TGF 1 and PTEN promoter. Data are shown as . ns: nonsignificant, , , .

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