Possible Involvement of DNA Methylation in TSC1 Gene Expression in Neuroprotection Induced by Hypoxic Preconditioning

Figure 3

HPC upregulated the expression of TSC1 and enhanced the activity of autophagy in the hippocampal CA1 region. (a) TSC1 mRNA. (b) TSC2 mRNA. (c) mTOR mRNA. (d) LC3 mRNA. (e) Typical western blot pictures of TSC1, TSC2, mTOR, P-mTOR, P-p70S6K, and LC3-II/LC3-I ratio after exposed to HPC. (f) The relative protein levels of TSC1 in the control, H1, and HPC groups. (g) The relative protein levels of TSC2 in the control, H1, and HPC groups. (h) The relative protein levels of mTOR in the control, H1, and HPC groups. (i) The relative protein levels of P-mTOR (Ser2448) in the control, H1, and HPC groups. (j) The relative protein levels of P-p70S6K (Thr 389) in the control, H1, and HPC groups. (k) The relative protein levels of LC3-II/LC3-I ratio in the control, H1, and HPC groups. (l) The fluorescence intensity of TSC1 in hippocampus of mice. (m) The histogram of TSC1 fluorescence value analysis between the CA1 and CA3 areas in the control group, H1 group, and hypoxic preconditioning group (, vs. control CA1; ^#, ^## vs. control CA3, per group,  μm).

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