Possible Involvement of DNA Methylation in TSC1 Gene Expression in Neuroprotection Induced by Hypoxic Preconditioning

Figure 4

The methylation changes of CpG sites in TSC1 promoter region (-720 bp~ -360 bp) were detected by bisulfite-modified DNA sequencing (BMDS). Ten single clones are represented for each sample. The CpG island is depicted, and each circle illustrates a single CpG. Closed circles represent methylated CpG, whereas open circles represent unmethylated CpG.