Possible Involvement of DNA Methylation in TSC1 Gene Expression in Neuroprotection Induced by Hypoxic Preconditioning

Figure 5

HPC induced the TSC1 expression and the activity of mTOR/p70S6K/autophagy pathway with the change of ATP consumption and ROS production in HT22 cells. (a) The relative mRNA abundance of TSC1, TSC2, mTOR, p70S6K, and LC3. (b) Typical western blot pictures of TSC1, TSC2, mTOR, P-mTOR (Ser2448), P-p70S6K (Thr389), and LC3-II/LC3-I ratio protein levels in HT22 cells. (c) The relative protein levels of TSC1, TSC2, mTOR, P-mTOR (Ser2448), P-p70S6K (Thr389), and LC3-II/LC3-I ratio in the control, H1, and HPC groups. (d) The fluorescence intensity of TSC1 in HT22 cells in the control group, H1 group, and HPC group. (e) The histogram of TSC1 fluorescence value analysis in the control, H1, and HPC groups. (f) The pictures of pe-GFP-LC3-II in the control, H1, and HPC groups. (g) The histogram of pe-GFP-LC3-II in the control, H1, and HPC groups. (h) Relative concentration of ATP in the control, H1, and HPC groups of HT22 cells. (i) Relative concentration of ROS in the control, H1, and HPC groups of HT22 cells (, vs. the control group, ^# vs. the H1 group, per group,  μm).

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