Possible Involvement of DNA Methylation in TSC1 Gene Expression in Neuroprotection Induced by Hypoxic Preconditioning

Figure 6

TSC1 contributes to cell viability and apoptosis, which is related to ATP consumption and ROS production under hypoxia conditions in HT22 cells. (a) Overexpression of the TSC1 gene could increase the cell viability in HT22 cells when exposed to hypoxia. (b) Knockout of TSC1 gene in HT22 cells could decrease the cell viability in HT22 cells when exposed to hypoxia. (c) Overexpression of the TSC1 gene could reduce the cell apoptosis in HT22 cells when exposed to hypoxia. (d) The histogram of apoptosis of overexpressed TSC1. (e) Knockout of the TSC1 gene could increase the cell apoptosis in HT22 cells when exposed to hypoxia. (f) The histogram of apoptosis of knockdown TSC1. (g) Overexpressed TSC1 gene upregulated ATP concentration in HT22 cells when exposed to hypoxia. (h) Overexpressed TSC1 gene could decrease ROS concentration in HT22 cells when exposed to hypoxia. (i) Knockout of TSC1 gene could downregulate ATP concentration in HT22 cells when exposed to hypoxia in HT22 cells. (j) Knockout of TSC1 gene could increase ROS concentration in HT22 cells when exposed to hypoxia in HT22 cells (, vs. the vector group).

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