Possible Involvement of DNA Methylation in TSC1 Gene Expression in Neuroprotection Induced by Hypoxic Preconditioning

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Primer</td><td class="align_center">Forward 5<svg height="7.86364pt" id="M6" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg> →3<svg height="7.86364pt" id="M7" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg></td><td class="align_center">Reverse 5<svg height="7.86364pt" id="M8" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg> →3<svg height="7.86364pt" id="M9" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg></td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">TSC1-EGFP-N1</td><td class="align_center">TGCAGCTAGCATGGCCCAACAAGCAAAT</td><td class="align_center">CTAGGGTACCATGCTGTGTTCATGATGAGT</td></tr><tr><td class="align_left">CRISPR-Cas9-TSC1-px330-EGFP</td><td class="align_center">CACCGTCTTTGGCCGTCTCTCGTCA</td><td class="align_center">CAGAAACCGGCAGAGAGCAGTCAAA</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Primer sequences of clone and CRISPR-Cas9.</div>

Oxidative Medicine and Cellular Longevity

tab2

Table 2

Table 2: Possible Involvement of DNA Methylation in TSC1 Gene Expression in Neuroprotection Induced by Hypoxic Preconditioning 